Preparation of Extracellular Matrices Produced by Cultured and Primary Fibroblasts
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چکیده
منابع مشابه
Secretion and extracellular processing of procollagen by cultured human fibroblasts.
Cultures of human diploid fibroblasts were labeled with radioactive proline and glycine, and the precursor of collagen (procollagen) in cells and medium was characterized by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. A covalently assembled molecule with the composition (pro alpha1)(2).pro alpha2 (approximate molecular weight, 360,000) appeared intracellularly soon after synthesi...
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Penicilliurn expamum grown in a medium with rice husk as a carbon source produced an extracellular protease. The protease enzyme was isolated from culture broth by fractionation with acetone and column chromatography on Sephadex G- 100 and DEAE A-50. The protease enzyme was purified about 17.47 fold, with a recovery of 14%. The purified protease was homogenous on SDS polyacrylarnide disc ge...
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ژورنال
عنوان ژورنال: Current Protocols in Cell Biology
سال: 2006
ISSN: 1934-2500,1934-2616
DOI: 10.1002/0471143030.cb1009s33